Detection and Genotyping of Hepatitis D Virus from HBsAg Positive Patients in Iran Using RT-PCR
نویسندگان
چکیده مقاله:
Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequences corresponding to the Hepatitis delta antigen (HDAg) C-termini were used for genotyping. The results showed that 63.6% (23 of 36) of (HDAb) positive serum samples (as determined by ELISA) were also positive for HDV-RNA. Sequencing and phylogenic analysis of three Iranian HDV isolates revealed the most homology (93%) with an Italian isolate indicating a close relationship and probably a common origin for these isolates.
منابع مشابه
detection and genotyping of hepatitis d virus from hbsag positive patients in iran using rt-pcr
hepatitis delta virus (hdv) is a degenerate rna virus or virusoid and a satellite of hepatitis b virus (hbv). three distinct genotypes are described for hdv; genotype i is distributed worldwide but other genotypes appear to be more restricted geographically. in the present study, an rt-nested pcr method was set up to detect delta infection from serum samples. moreover, the target amplified sequ...
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عنوان ژورنال
دوره 4 شماره 3
صفحات 174- 179
تاریخ انتشار 2006-07-01
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